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All rights reserved. © Copyright 2001, Applied Biosystems. All rights reserved. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document. Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the U.S. and certain other countries. AB (Design), Applera, Biospectrometry, CombiSolv, Data Explorer, Mariner, and Voyager are trademarks of Applera Corporation or its subsidiaries in the U.S. and certain other countries. HP and Laserjet are registered trademarks of Hewlett-Packard Co. Microsoft, PowerPoint, Visual Basic, and Windows NT are registered trademarks of Microsoft Corporation. All other trademarks are the sole property of their respective owners. Printed in the USA, 7/2001 Part Number 4317717 Rev. C Table of Contents Table of Contents How to Use This Guide .............................................................. xi Chapter 1 Data Explorer™ Basics 1.1 Overview .............................................................................. 1-2 1.2 File Formats and Types ............................................................ 1-5 1.2.1 Software Applications Compatibility ....................................... 1-5 1.2.2 Data (.DAT) File Format ......................................................... 1-5 1.3 Parts of the Data Explorer Window............................................. 1-11 1.4 Customizing the Data Explorer Window ....................................... 1-17 1.4.1 Setting Default Values ...........................................................1-17 1.4.2 Customizing Processing and Graphic Settings (.SET) ...........1-17 1.4.3 Customizing Toolbars ............................................................1-21 1.5 Setting Graphic Options .......................................................... 1-23 1.5.1 Changing Background Color .................................................1-23 1.5.2 Customizing Graphic Options ................................................1-24 1.5.3 Reverting to Previous Graphic Options .................................1-29 1.6 Managing Files...................................................................... 1-30 1.6.1 Converting .SPC File Format to .DAT File Format (Mariner Data Only) ..............................................................1-30 1.6.2 Converting Data from Profile to Centroid (Mariner Data Only) ..............................................................1-33 1.6.3 Converting to and Exporting ASCII Data ..............................1-34 1.6.4 Importing a Trace in ASCII Format ........................................1-35 1.6.5 Extracting and Saving Information from .DAT, .RSD, and .RCD Files ..........................................1-36 1.6.6 Copying from Data Files ........................................................1-38 Data Explorer™ Software User’s Guide Table of Contents Table of Contents 2.1 Opening and Closing Data Files ................................................. 2-2 2.1.1 Opening Data Files ................................................................ 2-2 2.1.2 Displaying Mariner DAD Traces ............................................. 2-6 2.1.3 Displaying Voyager Chromatograms ...................................... 2-7 2.1.4 Viewing Read-Only Files ........................................................ 2-7 2.1.5 Moving Between Open Files .................................................. 2-8 2.1.6 Closing Data Files .................................................................2-10 2.2 Adjusting the Display Range..................................................... 2-11 2.3 Organizing Windows............................................................... 2-13 2.4 Manipulating Traces ............................................................... 2-14 2.4.1 Zooming, Centering, and Customizing a Trace ......................2-14 2.4.2 Duplicating a Trace ...............................................................2-15 2.4.3 Dividing the Active Trace ......................................................2-15 2.4.4 Adding Traces from the Same Data File to a Window ...........................................2-16 2.4.5 Removing Traces ..................................................................2-21 2.4.6 Expanding and Linking Traces ..............................................2-21 2.4.7 Recalling and Rearranging Traces (Processing History) ........2-22 2.4.8 Overlaying Traces .................................................................2-24 2.4.9 Annotating Traces .................................................................2-28 2.4.10 Viewing Trace Labels .......................................................