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All rights reserved. Agilent® is a registered trademark of Agilent Technologies Inc. Hewlett-Packard® is a registered trademark of Hewlett-Packard Company. LCQ™, LTQ™, LXQ™, MSQ™, TSQ™, and Xcalibur™ are trademarks of Thermo Fisher Scientific Inc. Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser. Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error- free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly. This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Release history: First release February 2007, second release July 2007 For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug Administration or other competent authorities. C C Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . .v About This Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi Chapter 1 Agilent Modules Supported by LC Devices Versions 2.0.2 and 2.1.0. . . . . . . . . . .1 Chapter 2 Connecting the Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Ethernet Communication Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Installing a Network Card in an Agilent Module. . . . . . . . . . . . . . . . . . . . . . . . .4 Connecting the Ethernet Communication Cables. . . . . . . . . . . . . . . . . . . . . . . .7 Installing the External Contact Interface Board.. . . . . . . . . . . . . . . . . . . . . . . . .8 Connecting the Trigger Cable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Chapter 3 Configuring the Autosampler for Contact Closure . . . . . . . . . . . . . . . . . . . . . . . . .11 Chapter 4 Triggering Data Acquisition with the Autosampler. . . . . . . . . . . . . . . . . . . . . . . .13 Chapter 5 Turning Off the Solvent Tracking Feature.. . ... . . . . . . . . . . . . . . . . . . . . . . . . . . .15 Appendix A Upgrading an HP 1100 Series LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition P P Preface About This Guide This guide describes how to hardwire an Agilent® 1100 Series LC to a Thermo Scientific mass spectrometer and the data system computer, configure an Agilent autosampler for contact closure from within the Xcalibur Instrument Configuration program, and trigger data collection with an Agilent autosampler. This guide also describes how to upgrade a Hewlett-Packard® 1100 Series LC to an Agilent 1100 Series LC with Ethernet communication. Related Documentation In addition to this guide, Thermo Fisher Scientific provides Help available from within the Xcalibur software. Safety and Special Notices Make sure you follow the precautionary statements presented in this guide. The safety and other special notices appear in boxes. Safety and special notices include the following: CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION notice is accompanied by an appropriate CAUTION symbol. CAUTION Highlights electric shock related hazards to human beings. Each electric shock notice is accompanied by the international High Voltage symbol. IMPORTANT Highlights information necessary to prevent damage to software, loss of data, or invalid test results; o...